Biochemical, Phytochemical and Antioxidant Analysis of Leaves and Seed of Fenugreek Plant
Keywords:Trigonella foenum graecum, Trigonella corniculata, Biochemical, Phytochemical, antioxidants, DPPH, Phenolic acids
In the present study leaves and seeds of two different varieties of Fenugreek plant viz. Trigonella foenum gracium and Trigonella corniculata, collected from Bahawalpur, Pakistan were subjected to the biochemical, phytochemical and antioxidant analysis. The content of total sugars, non reducing sugars and reducing sugars in selected parts of these varities were found to be 5.007±0.101 to 8.133±0.223, 4.255±0.150 to 7.099±0.316 and 0.751±0.064 to 1.410±0.071 g/100g of dry weight respectively. Water soluble, salt soluble and total salt soluble protein content range from 1.172±0.001 to 6.015±0.132, 1.517±0.038 to 1.174±0.006 and 0.013±0.001 to 0.018±0.001 g/100 g of dry weight respectively and the total free amino acids ranged from 1.198±0.014 to 4.554±0.006 g/100 g of dry weight. The range of tannins and saponin contents in analyzed parts of these varities was found to be 0.057±0.009 to 0.117±0.025 and 0.083±0.012 to 0.0151±0.045 g /100g of dry weight respectively. Similarly, flavonoids and hydrogen cyanide content ranged from 0.021±0.000 to 0.055±0.004 and 0.0234±0.008 to 0.307±0.009 g/100g of dry weight respectively; while ascorbic acid content was found to be in the range of 0.068±0.08 to 0.267±0.031 g/100g of dry weight. Total phenolic acid content of the selected parts of these varities were estimated on the basis of difference in dipole moment and its range was observed from 0.0539±0.024 to 0.856±0.064 g/100g of dry weight. The total antioxidants analysis showed that ascorbic acid and trolox contents (mg Eqv./100g of extract) were found to be lower in Trigonella corniculata seeds (0.066±0.001 and 0.047±0.001 mg Eqv./100g respectively) and higher in Trigonella corniculata leaves (0.069±0.001 and 0.050±0.001 mg Eqv./100g respectively).The DPPH radical scavenging activity and reducing power were found to be in the range of 31.736±2.430 to 39.590±2.432 and 0.974±0.231 to 2.301±0.068 respectively. The different parts of studied varities of fenugreek plant, having higher values of %DPPH inhibition and reducing power showed least count of IC50 and vice versa.
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