Decoding Genetic Signatures for Identification: A Molecular Investigation Approach in Pakistan via Cytochrome C Oxidase I (COI) Gene Sequencing

Authors

  • Muhammad Usman Department of Zoology, The university of Lahore, Sargodha campus, Sargodha, Punjab, Pakistan
  • Sahrish Ghazanfar Department of Zoology, The University of Lahore, Sargodha Campus, Sargodha, Punjab, Pakistan
  • Sana Ashraf Department of Zoology, The University of Lahore, Sargodha Campus, Sargodha, Punjab, Pakistan
  • Asma Iqbal Department of Zoology, The University of Lahore, Sargodha Campus, Sargodha, Punjab, Pakistan
  • Naila Amjad Department of Zoology, The University of Lahore, Sargodha Campus, Sargodha, Punjab, Pakistan
  • Shakeel Ahmad Institute of Zoology, Bahauddin Zakariya University, Multan, Punjab, Pakistan
  • Sahrish Naqvi Institute of Zoology, Bahauddin Zakariya University Multan, Punjab, Pakistan
  • Muhammad Khalid Institute of Zoology, Bahauddin Zakariya University Multan, Punjab, Pakistan
  • Ayesha Imtiaz Institute of Zoology, Bahauddin Zakariya University Multan, Punjab, Pakistan
  • Mudassar Maqbool Department of Zoology, Islamia University of Bahawalpur, Punjab, Pakistan
  • Muhammad Imran Department of Statistics, Bahaudin Zakariya University, Multan, Punjab, Pakistan.
  • Hassan Raza Javed Department of Botany, Govt Graduate College Layyah, Punjab, Pakistan
  • Sana Shahid Department of Biology, Govt Graduate College for Women, Khanewal, Punjab, Pakistan

DOI:

https://doi.org/10.52700/fcs.v5i1.73

Keywords:

Identification, Fish, Cytochrome C Oxidase I, Pakistan, Sequencing

Abstract

DNA barcoding is an efficient and rapid approach to the identification of species. A standard genetic marker is used to barcode the sequence of unidentified species. So, mitochondrial cytochrome c oxidase I (COI) genetic marker is mostly used for species molecular identification. Therefore, molecular identification and phylogeographic relationship of Cirrhinus reba (C. reba) were carried out in the present study. In the present study, we propose the barcoding analysis of the mitochondrial cytochrome c oxidase I (COI) gene of freshwater fish as different sequences were obtained from the sequencer and found (all < 500) base pairs and successfully barcode with mitochondrial COI genetic marker for identification. BLAST analysis provides 100% identity match with reference sequences of GenBank database and confirmed the species as C. reba as the length of the sequence is found 496 base pairs. The present study successfully reported DNA barcoding for genetic diversity and identification contributing to covering sufficient information about molecular analysis to identify unknown species the best of our knowledge this is the first study reporting a genetic tool for identification of the commercial food fish of Pakistan. Moreover, DNA barcoding can play a very significant role in molecular identification and inference of relationships in diverse freshwater ecosystem.

Published

2024-06-30

How to Cite

Muhammad Usman, Sahrish Ghazanfar, Sana Ashraf, Asma Iqbal, Naila Amjad, Shakeel Ahmad, Sahrish Naqvi, Muhammad Khalid, Ayesha Imtiaz, Mudassar Maqbool, Muhammad Imran, Hassan Raza Javed, & Sana Shahid. (2024). Decoding Genetic Signatures for Identification: A Molecular Investigation Approach in Pakistan via Cytochrome C Oxidase I (COI) Gene Sequencing. FRONTIERS IN CHEMICAL SCIENCES, 5(1), 12-17. https://doi.org/10.52700/fcs.v5i1.73